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Concentration and Enzyme Content of In Vitro‐Cultured Babesia bigemina ‐Infected Erythrocytes 1

1986; Wiley; Volume: 33; Issue: 4 Linguagem: Inglês

10.1111/j.1550-7408.1986.tb05653.x

2375-0804

Carlos Agustín Vega, Gerald Μ. Buening, Sergio D. Rodríguez, C. A. Carson,

Mosquito-borne diseases and control

ABSTRACT. Clones of in vitro‐cultured Babesia bigemina ‐infected erythrocytes were concentrated by several density gradient procedures. The density range of infected erythrocytes containing pairs of parasites was 1.077 to 1.089 g/ml, whereas the density range of infected erythrocytes containing single parasites was 1.092 to 1.100 g/ml. Three enzymes‐lactate dehydrogenase, glucose‐phosphate isomerase, and glutamate dehydrogenase‐were found associated with infected erythrocytes. The parasite‐specific enzyme and/or isoenzymes were shown to have different mobility patterns in starch gel electrophoresis from those found in the normal bovine erythrocytes. The enzyme 6‐phosphogluconate dehydrogenase was not detected as a parasite‐specific enzyme in B. bigemina ‐infected erythrocytes.