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Effects of protein incorporation on calcium phosphate coating

2008; Elsevier BV; Volume: 29; Issue: 3 Linguagem: Inglês

10.1016/j.msec.2008.08.003

1873-0191

Isabel B. Leonor, C. M. Alves, Helena S. Azevedo, Rui L. Reis,

Dental materials and restorations

The incorporation of proteins into calcium phosphate (Ca-P) coatings is expected to alter their properties.The aim of this work is, therefore, to study the effect of protein concentration on the formation of Ca-P film.A biodegradable blend of corn starch/ethylene vinyl alcohol (SEVA-C) was used as substrate and bioactive glass (45S5 Bioglass ® ) was used as a nucleating agent.Bovine serum albumin (BSA) and α-amylase were added, separately, at a concentration of 0.5, 1, and 5 mg/mLto simulated body fluid (SBF) solutions, at the nucleation stage.The incorporation of protein molecules was shown to affect the properties of Ca-P coatings in terms of morphology, composition and crystallinity.Both proteins seem to inhibit in some extent and/or retard the growth of Ca-P nuclei at 0.5 and 5 mg/mL concentrations.FTIR analyses revealed the presence of phosphate and carbonate groups, confirming the formation of a Ca-P layer.The characteristic groups of protein molecules were also detected on the IR spectra, which indicate the efficient incorporation of the proteins into the coatings.When α-amylase was added to the SBF solution the production of reducing sugars was detected, proving the retention of enzyme activity.These results suggest the carrier potential of Ca-P coatings for the sustained delivery of other biologically active proteins and consequently with a strong potential for inducing bone tissue regeneration.