Hyperexplexia or stiff baby syndrome.
1991; BMJ; Volume: 66; Issue: 4 Linguagem: Inglês
10.1136/adc.66.4.460
ISSN1468-2044
AutoresC Tohier, Jean‐Christophe Rozé, Anthony S. David, M F Veccierini, P. Renaud, A Mouzard,
Tópico(s)RNA Research and Splicing
ResumoAbstract CRISPR guide-RNA libraries have been iteratively optimised to provide increasingly efficient reagents although their large size is a barrier for some applications. We designed a minimal genome-wide human CRISPR-Cas9 library (MinLibCas9), optimised by mining existing large-scale gene loss-of-function datasets, resulting in a greater than 46% reduction in size compared to other libraries while preserving assay sensitivity and specificity. MinLibCas9 improves the efficiency of CRISPR-Cas9 loss-of-function screens and expands their application to complex models and assays.
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