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Development and validation of a diagnostic test for Ridge allele copy number in Rhodesian Ridgeback dogs

2015; BioMed Central; Volume: 2; Issue: 1 Linguagem: Inglês

10.1186/s40575-015-0013-x

2052-6687

Jennifer Turner Waldo, Kasandra Santana Diaz,

Animal Genetics and Reproduction

The breed-defining dorsal ridge in Rhodesian Ridgeback dogs is the result of a 133,000 base pair duplication on chromosome 18. Because this trait is dominant, heterozygous dogs cannot be discriminated from those with two copies of the Ridge allele. A quantitative PCR test was developed and dogs of known genotype were used as test subjects. In all cases, the correct genotype was determined experimentally. This work provides a rapid and accurate methodology for determining dog genotype with respect to the Ridge allele.