Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip
2008; Royal Society of Chemistry; Volume: 8; Issue: 4 Linguagem: Inglês
10.1039/b716388f
ISSN1473-0197
AutoresLouis P. Hromada, Brian J. Nablo, John J. Kasianowicz, Michael Gaitan, Don L. DeVoe,
Tópico(s)Microfluidic and Capillary Electrophoresis Applications
ResumoThe measurement of single poly(ethylene glycol) (PEG) molecules interacting with individual bilayer lipid membrane-bound ion channels is presented. Measurements were performed within a polymer microfluidic system including an open-well bilayer lipid membrane formation site, integrated Ag/AgCl reference electrodes for on-chip electrical measurements, and multiple microchannels for independent ion channel and analyte delivery. Details of chip fabrication, bilayer membrane formation, and alpha-hemolysin ion channel incorporation are discussed, and measurements of interactions between the membrane-bound ion channels and single PEG molecules are presented.
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