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Trypanosoma cruzi: Immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes

2008; Elsevier BV; Volume: 120; Issue: 3 Linguagem: Inglês

10.1016/j.exppara.2008.08.001

1090-2449

Wendel Coura‐Vital, Cláudia Martins Carneiro, Helen Rodrigues Martins, Marta de Lana, Vanja Maria Veloso, Andréa Teixeira−Carvalho, Maria Terezinha Bahia, Rodrigo Corrêa‐Oliveira, O. A. Martins‐Filho, Wagner Luiz Tafuri, Alexandre Barbosa Reis,

Research on Leishmaniasis Studies

A detailed investigation has been carried out about the serological profiles of groups of dogs experimentally infected with metacyclic (MT) or blood (BT) trypomastigotes of Berenice-78 Trypanosoma cruzi strain. Peripheral blood was collected from infected dogs and uninfected controls, weekly during 35 days following the acute phase of infection, and immunoglobulin profiles were determined by ELISA. Dogs infected with BT exhibited unaltered levels of IgG2, increases in IgM, IgE, IgA, IgG and IgG1. In contrast, dogs infected with MT presented unaltered levels of IgE and IgG1 and an increase in IgM, IgA, IgG and IgG2 levels. Compared with the MT group, animals infected with BT showed significant increases in IgM on days 7, 14 and 28, in IgA on days 7, 14 and 21, in IgE on days 7 and 14, in IgG on days 14 and 28, and in IgG1 on days 7, 14 and 21. Parasitemia levels of the infected animals were measured over the same time period. No correlations were found between the immunoglobulin profiles and the parasitemia levels. The results demonstrated that the inoculum source (BT or MT) influence the immunoglobulin isotype profile that may drive distinct outcome of acute canine Chagas disease.