Ca 2+ ‐induced Ca 2+ release in insulin‐secreting cells
1992; Wiley; Volume: 296; Issue: 3 Linguagem: Inglês
10.1016/0014-5793(92)80306-2
ISSN1873-3468
AutoresMd. Shahidul Islam, Patrik Rorsman, Per‐Olof Berggren,
Tópico(s)Adenosine and Purinergic Signaling
ResumoThe sulphydryl reagent thimerosal (50 μM) released Ca 2+ from a non‐mitochondrial intracellular Ca 2+ pool in a dose‐dependent manner in permeabilized insulin‐secreting RINm5F cells. This release was reversed after addition of the reducing agent dithiothreitol, Ca 2+ was released from an Ins(1,4,5)P 3 ‐insensitive pool, since release was observed even after depletion of the Ins(1,4,5)P 3 ‐sensitive pool by a supramaximal dose of Ins(2,4,5)P 3 or thapsigargin. The Ins(1,4,5)P 3 ‐sensitive pool remained essentially unaltered by thimerosal. Thimerosal‐induced Ca 2+ release was potentiated by caffeine. These findings suggest the existence of Ca 2+ ‐induced Ca 2+ release also in insulin‐secreting cells.
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