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Curtobacterium ammoniigenes sp. nov., an ammonia-producing bacterium isolated from plants inhabiting acidic swamps in actual acid sulfate soil areas of Vietnam

2007; Microbiology Society; Volume: 57; Issue: 7 Linguagem: Inglês

10.1099/ijs.0.64731-0

1466-5034

Tomoko Aizawa, Nguyen Bao Ve, Ken-ichiro Kimoto, Noriyuki Iwabuchi, Hiroaki Sumida, Isao Hasegawa, Satohiko Sasaki, Tomohiko Tamura, Takuji Kudo, Ken‐ichiro Suzuki, Mutsuyasu Nakajima, Michio Sunairi,

Microbial Natural Products and Biosynthesis

The ammonia-producing bacteria B55 T , CA73, SA69 and SA72 were isolated from the waterweeds Ludwigia adscendens (B55 T ) and Eleocharis dulcis (CA73, SA69 and SA72) grown in highly acidic swamps (pH 2–4) in actual acid sulfate soil areas of Vietnam. The isolates were Gram-positive, irregular rod-shaped, non-spore-forming bacteria. On the basis of 16S rRNA gene sequence similarity, strain B55 T was shown to belong to the genus Curtobacterium of the class Actinobacteria . Chemotaxonomic data (MK-9 as major isoprenoid quinone, d -ornithine as cell-wall diamino acid, acetyl as the acyl type of peptidoglycan) supported the affiliation of all four strains to this genus. Although their 16S rRNA gene sequence similarity was 99 % to species with validly published names within the genus, they formed a group that was distinct in the phylogenetic tree, and DNA–DNA relatedness values to these established species were less than 10 %. The results of physiological and biochemical tests and major fatty acids (cyclohexyl-C 17 : 0 , anteiso-C 17 : 0 and cyclohexyl-C 19 : 0 ) allowed phenotypic differentiation of these strains from the species of Curtobacterium with validly published names. Therefore, strains B55 T , CA73, SA69 and SA72 represent a novel species, for which the name Curtobacterium ammoniigenes sp. nov. is proposed. The type strain is B55 T (=NBRC 101786 T =VTCC D6-11 T =JCM 14609 T ).