Portal de Periódicos da CAPES

The murine IgG 1 /IgE class switch program

1992; Wiley; Volume: 22; Issue: 7 Linguagem: Inglês

10.1002/eji.1830220723

1521-4141

Gregor Siebenkotten, Charlotte Esser, Andreas Radbruch, Matthias Wabl,

Immune Cell Function and Interaction

Abstract Immunoglobulin class switching is controlled by cytokines. Thus, interleukin‐4 (IL‐4) directs class switching to both IgG 1 and IgE. Consistent with this are the results reported here on restriction endonuclease analysis of active and inactive alleles of the IgH locus in IgE‐producing cells. In cells that were stimulated in vitro by lipopolysaccharide and IL‐4 the silent alleles preferentially switched to γ 1 , whereas in cells that were stimulated by antigen in vivo both active and inactive alleles switched to γ1. Thirty percent of the recombined switch regions (S μ /S ϵ ) contain S γ 1 sequences, which we interpret as footprints of a previous switch to γ 1 . Since this percentage is a minimum estimate, between 30% and 100% of switching to ϵ must occur sequentially via γ 1 .