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Modeling Inducible Human Tissue Neoplasia Identifies an Extracellular Matrix Interaction Network Involved in Cancer Progression

2009; Cell Press; Volume: 15; Issue: 6 Linguagem: Inglês

10.1016/j.ccr.2009.04.002

1878-3686

Jason Reuter, Susana Ortiz‐Urda, Markus Kretz, John Garcia, Florence A. Scholl, Anna M.G. Pasmooij, David S. Cassarino, Howard Y. Chang, Paul A. Khavari,

Gene expression and cancer classification

To elucidate mechanisms of cancer progression, we generated inducible human neoplasia in three-dimensionally intact epithelial tissue. Gene expression profiling of both epithelia and stroma at specific time points during tumor progression revealed sequential enrichment of genes mediating discrete biologic functions in each tissue compartment. A core cancer progression signature was distilled using the increased signaling specificity of downstream oncogene effectors and subjected to network modeling. Network topology predicted that tumor development depends on specific extracellular matrix-interacting network hubs. Blockade of one such hub, the β1 integrin subunit, disrupted network gene expression and attenuated tumorigenesis in vivo. Thus, integrating network modeling and temporal gene expression analysis of inducible human neoplasia provides an approach to prioritize and characterize genes functioning in cancer progression.