Chemical Synthesis of Lymphotactin: A Glycosylated Chemokine with a C-Terminal Mucin-Like Domain
2001; Wiley; Volume: 7; Issue: 5 Linguagem: Inglês
10.1002/1521-3765(20010302)7
ISSN1521-3765
AutoresLisa A. Marcaurelle, Laura S. Mizoue, Jill Wilken, Lisa Oldham, Stephen B. H. Kent, Tracy M. Handel, Carolyn R. Bertozzi,
Tópico(s)Chemical Synthesis and Analysis
ResumoThe synthesis of a 93-residue chemokine, lymphotactin, containing eight sites of O-linked glycosylation, was achieved using the technique of native chemical ligation. A single GalNAc residue was incorporated at each glycosylation site using standard Fmoc-chemistry to achieve the first total synthesis of a mucin-type glycoprotein. Using this approach quantities of homogeneous material were obtained for structural and functional analysis.
Referência(s)