Altered Cytoskeletal Structure of Smooth Muscle Cells in Ureteropelvic Junction Obstruction
2011; Lippincott Williams & Wilkins; Volume: 185; Issue: 6 Linguagem: Inglês
10.1016/j.juro.2011.02.045
ISSN1527-3792
AutoresGiuseppina Cutroneo, Salvatore Arena, Giuseppe Anastasi, Raimondo M. Cervellione, Silvia Grimaldi, Debora Di Mauro, Francesco Speciale, Francesco Arena, Vincenzo Di Benedetto, Angelo Favaloro, Carlo Magno,
Tópico(s)Hernia repair and management
ResumoNo AccessJournal of UrologyPediatric Urology1 Jun 2011Altered Cytoskeletal Structure of Smooth Muscle Cells in Ureteropelvic Junction Obstruction Giuseppina Cutroneo, Salvatore Arena, Giuseppe Anastasi, Raimondo M. Cervellione, Silvia Grimaldi, Debora Di Mauro, Francesco Speciale, Francesco Arena, Vincenzo Di Benedetto, Angelo Favaloro, and Carlo Magno Giuseppina CutroneoGiuseppina Cutroneo Department of Biomorphology and Biotechnology, University of Messina, Messina, Italy , Salvatore ArenaSalvatore Arena Unit of Urology, University of Messina, Messina, Italy Department of Pediatric Urology, New Royal Manchester Children's Hospital, Manchester, United Kingdom , Giuseppe AnastasiGiuseppe Anastasi Department of Biomorphology and Biotechnology, University of Messina, Messina, Italy , Raimondo M. CervellioneRaimondo M. Cervellione Department of Pediatric Urology, New Royal Manchester Children's Hospital, Manchester, United Kingdom , Silvia GrimaldiSilvia Grimaldi Unit of Pediatric Surgery, University of Catania, Catania, Italy , Debora Di MauroDebora Di Mauro Department of Biomorphology and Biotechnology, University of Messina, Messina, Italy , Francesco SpecialeFrancesco Speciale Department of Biomorphology and Biotechnology, University of Messina, Messina, Italy , Francesco ArenaFrancesco Arena Unit of Pediatric Surgery, University of Messina, Messina, Italy , Vincenzo Di BenedettoVincenzo Di Benedetto Unit of Pediatric Surgery, University of Catania, Catania, Italy , Angelo FavaloroAngelo Favaloro Department of Biomorphology and Biotechnology, University of Messina, Messina, Italy , and Carlo MagnoCarlo Magno Unit of Urology, University of Messina, Messina, Italy View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.045AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Ureteropelvic junction obstruction is one of the most common causes of hydronephrosis in children. A malfunction of smooth muscle cells is believed to be the underlying mechanism causing obstruction. We investigated the expression of some integrins, talin and β-dystroglycan, considered the main compound of smooth muscle cell cytoskeleton, and active caspase 3 at the level of the ureteropelvic junction obstruction. Materials and Methods: Specimens were obtained at pyeloplasty in 12 children with ureteropelvic junction obstruction. Six control specimens were obtained during organ explantation. Specimens were divided into renal pelvis, ureteropelvic junction and ureter below the obstruction. Western blot analysis of active caspase 3, and immunofluorescence and polymerase chain reaction analysis were performed for α7A, β1A, α7B and β1D integrins, talin and β-dystroglycan. Results: Talin and β-dystroglycan were slightly impaired in ureteropelvic junction obstruction, while α7B and β1D integrins were severely reduced, and α7A, β1A and active caspase 3 were significantly enhanced compared to controls. Conclusions: We demonstrated activation of apoptosis and a critical alteration of cytoskeleton that might explain the altered function and the increased apoptosis in smooth muscle cells in ureteropelvic junction obstruction. The delayed rearrangement of the cytoskeleton of smooth muscle cells in ureteropelvic junction obstruction might be linked to a postnatal splicing from α7A and β1A to α7B and β1D integrins, respectively. This relationship could explain the common clinical scenario of spontaneous improvement of hydronephrosis in children with suspected ureteropelvic junction obstruction. References 1 : The roles of extracellular matrix proteins, apoptosis and c-kit positive cells in the pathogenesis of ureteropelvic junction obstruction. 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Google Scholar © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 6June 2011Page: 2314-2319 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.Keywordssmooth muscleintegrinsureteral obstructionmyocytescytoskeletontalinMetricsAuthor Information Giuseppina Cutroneo Department of Biomorphology and Biotechnology, University of Messina, Messina, Italy More articles by this author Salvatore Arena Unit of Urology, University of Messina, Messina, Italy Department of Pediatric Urology, New Royal Manchester Children's Hospital, Manchester, United Kingdom More articles by this author Giuseppe Anastasi Department of Biomorphology and Biotechnology, University of Messina, Messina, Italy More articles by this author Raimondo M. Cervellione Department of Pediatric Urology, New Royal Manchester Children's Hospital, Manchester, United Kingdom More articles by this author Silvia Grimaldi Unit of Pediatric Surgery, University of Catania, Catania, Italy More articles by this author Debora Di Mauro Department of Biomorphology and Biotechnology, University of Messina, Messina, Italy More articles by this author Francesco Speciale Department of Biomorphology and Biotechnology, University of Messina, Messina, Italy More articles by this author Francesco Arena Unit of Pediatric Surgery, University of Messina, Messina, Italy More articles by this author Vincenzo Di Benedetto Unit of Pediatric Surgery, University of Catania, Catania, Italy More articles by this author Angelo Favaloro Department of Biomorphology and Biotechnology, University of Messina, Messina, Italy More articles by this author Carlo Magno Unit of Urology, University of Messina, Messina, Italy More articles by this author Expand All Advertisement PDF downloadLoading ...
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