Valproate Induces in Vitro Accumulation of Long-Chain Fatty Acylcarnitines
2001; Elsevier BV; Volume: 73; Issue: 4 Linguagem: Inglês
10.1006/mgme.2001.3200
ISSN1096-7206
AutoresM. F. B. Silva, Cornelis Jakobs, Marinus Durán, Isabel Tavares de Almeida, Ronald J. A. Wanders,
Tópico(s)Epilepsy research and treatment
ResumoTo elucidate the interference mechanisms of valproate (VPA) with mitochondrial fatty acid β-oxidation (FAO), the profile of acylcarnitine formation was studied in vitro. Human fibroblasts were incubated with 0.2 mmol/L [U-13C]palmitate, 0.4 mmol/L l-carnitine, ± VPA (2 mmol/L) (96 h at 37°C). Acylcarnitines (AC) were analyzed by GC-CI-MS. VPA induced an impaired production of acetylcarnitine (C2) and an increase on long-chain AC (C10 to C16) both in control and in FAO-deficient cell lines (VLCAD, LCHAD, MTP).
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