Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens
2012; BioMed Central; Volume: 6; Issue: 1 Linguagem: Inglês
10.1186/1752-153x-6-43
ISSN1752-153X
AutoresRahmat Ali Khan, Muhammad Rashid Khan, Sumaira Sahreen, Mushtaq Ahmed,
Tópico(s)Natural Antidiabetic Agents Studies
ResumoLaunaea procumbens (LP) has been used as a food supplement in Pakistan. In this study methanolic crude extract (LPME) of the whole plant and its different fractions; n-hexane (LPHE); ethyl acetate (LPEE) and chloroform (LPCE) were studied for the determination of total flavonoid and phenolics contents along with multifaceted in vitro scavenging assays. Considerable amount of flavonoid and phenolics contents were found in all the fractions. Methanol and chloroform fraction exhibited efficient scavenging of DPPH·, ABTS·+, ·OH, superoxide, lipid peroxide and nitric oxide free radicals. Significant correlation was found between DPPH·, ABTS·+, superoxide radical, β-carotene bleaching restraint and phosphomolybdenum assay with total flavonoids and phenolics contents. High performance chromatography (HPLC) of LPME revealed the presence of vitexin, orientin, rutin, hyperoside, catechin and myricetin. These results reveal the presence of bioactive compounds in LPME, which might be contributed towards the various in vitro scavenging.
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