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Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes

2012; UNIVERSIDADE DE SÃO PAULO; Volume: 48; Issue: 1 Linguagem: Inglês

10.1590/s1984-82502012000100012

2175-9790

Luciana Lopes Silva Pereira, Silvana Marcussi, Lívia Cabral Sátiro, Chrystian Araújo Pereira, L. F. D. B. de Andrade, Lisete Chamma Davide, Custódio Donizete dos Santos,

Plant Genetic and Mutation Studies

This study was conducted to evaluate the potential induction of genotoxic effects of white bean flour using the Comet assay. The test was conducted with human lymphocytes present in whole blood immediately after collection, by incubation with white bean flour in three concentrations (3.92, 9.52 and 18.18 mg/mL) at 37 ºC for 4 h followed by preparation of slides. Samples were considered positive (above 20% damage) when the damage observed to cellular DNA was higher than the negative control. No genotoxic potential was found at the doses tested. However, it would be premature to suggest absence of risk to human health of DNA damage since the exposure of cells to the extract was restricted to four hours rather than a whole cell cycle. Additionally, further information on toxicology should be obtained in future studies.