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Activation of archaeal transcription by recruitment of the TATA-binding protein

2003; National Academy of Sciences; Volume: 100; Issue: 9 Linguagem: Inglês

10.1073/pnas.0837150100

1091-6490

Mohamed Ouhammouch, Robert E. Dewhurst, Winfried Hausner, Michael Thomm, E. Peter Geiduschek,

RNA and protein synthesis mechanisms

The hyperthermophilic archaeon Methanococcus jannaschii encodes two putative transcription regulators, Ptr1 and Ptr2, that are members of the Lrp/AsnC family of bacterial transcription regulators. In contrast, this archaeon's RNA polymerase and core transcription factors are of eukaryotic type. Using the M. jannaschii high-temperature in vitro transcription system, we show that Ptr2 is a potent transcriptional activator, and that it conveys its stimulatory effects on its cognate eukaryal-type transcription machinery from an upstream activating region composed of two Ptr2-binding sites. Transcriptional activation is generated, at least in part, by Ptr2-mediated recruitment of the TATA-binding protein to the promoter.