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Comparison of a New Lateral-Flow Chromatographic Membrane Immunoassay to Viral Culture for Rapid Detection and Differentiation of Influenza A and B Viruses in Respiratory Specimens

2004; American Society for Microbiology; Volume: 42; Issue: 8 Linguagem: Inglês

10.1128/jcm.42.8.3661-3664.2004

1098-660X

Andreea C. Cazacu, Gail J. Demmler, M A Neuman, Betty A. Forbes, Soo-Young Chung, Jewel M. Greer, A. Álvarez, Robin Williams, Nadine Y. Bartholoma,

Viral gastroenteritis research and epidemiology

ABSTRACT The performance of a new rapid lateral-flow chromatographic membrane immunoassay test kit for detection of influenza virus was evaluated and compared to that of viral culture in respiratory secretions collected from 400 adults and children seen at three large university hospitals during the recent 2003 influenza season. The rapid test provided results in 15 min, with excellent overall performance statistics (sensitivity, 94.4%; specificity, 100%; positive predictive value, 100%; negative predictive value, 97.5%). Both influenza A and B type viruses were reliably detected, with no significant difference in performance statistics noted by influenza virus type or by the center performing the test.