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Transgenic Hydra allow in vivo tracking of individual stem cells during morphogenesis

2006; National Academy of Sciences; Volume: 103; Issue: 16 Linguagem: Inglês

10.1073/pnas.0510163103

1091-6490

Jörg Wittlieb, Konstantin Khalturin, Jan U. Lohmann, Friederike Anton‐Erxleben, Thomas C. G. Bosch,

Planarian Biology and Electrostimulation

Understanding the evolution of development in large part relies on the study of phylogenetically old organisms. Cnidarians, such as Hydra, have become attractive model organisms for these studies. However, despite long-term efforts, stably transgenic animals could not be generated, severely limiting the functional analysis of genes. Here we report the efficient generation of transgenic Hydra lines by embryo microinjection. One of these transgenic lines expressing EGFP revealed remarkably high motility of individual endodermal epithelial cells during morphogenesis. We expect that transgenic Hydra will become important tools to dissect the molecular mechanisms of development at the base of the Metazoan tree.