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Analysis of the Major Metabolite of 9-Tetrahydrocannabinol in Urine. IV. A Comparison of Five Methods

1984; Oxford University Press; Volume: 8; Issue: 6 Linguagem: Inglês

10.1093/jat/8.6.249

1945-2403

Alison Jones, Hala N. ElSohly, E. S. Arafat, Mahmoud A. ElSohly,

Alcohol Consumption and Health Effects

Urine samples from 29 subjects were simultaneously analyzed for 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid by five previously published methods. The procedures employed were radioimmunoassay (RIA), enzyme immunoassay (EIA), high performance liquid chromatography (HPLC), gas chromatography with electron capture detector (GC/ECD), and gas chromatography/mass spectrometry (GC/MS). A comparison of the results showed significant correlations between the results obtained by HPLC, GC/ECD, and GC/MS procedures. As anticipated, quantitation by any of these procedures resulted in lower values than those obtained by either of the immunoassays.