Aneuploidy and polyploidization in haploid tissue cultures of Larix decidua
1993; Wiley; Volume: 88; Issue: 1 Linguagem: Inglês
10.1111/j.1399-3054.1993.tb01762.x
ISSN1399-3054
AutoresPatrick von Aderkas, P.J. Anderson,
Tópico(s)Plant Reproductive Biology
ResumoHaploid embryogenic lines of Larix decidua which had been maintained on medium free of plant growth regulatiors were screened for chromosomal abnormalities. Chromosome squashes were prepared from fast‐growing suspension culture. alternatively, a method using burst protoplasts was devised for improving chromosome spreads. One line showed polyploidization with chromosome mumbers ranging from haploid to tetraploid. This line is presently predominantly diploid. Aneuploidization also occurred. The most effective method for counting chromosomes was from burst protoplasts. It is recommended that long‐term cultures of conifer tissues be checked for genetic stability.
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