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Isolation and characterization of polymorphic microsatellite loci in black sea bream ( Acanthopagrus schlegeli ) by cross-species amplification with six species of the Sparidae family

2007; EDP Sciences; Volume: 20; Issue: 3 Linguagem: Inglês

10.1051/alr

1765-2952

Yun‐Guo Liu, Lingxiao Liu, Zhenhua Wu, Hong Lin, Bafang Li, Xiuqin Sun,

Aquaculture Nutrition and Growth

We tested cross-species amplification of 68 existing microsatellite loci in 6 species of the Sparidae family: Acanthopagrus butcheri, Sparus aurata, Pagrus auratus, Chrysophrys major, Pagellus bogaraveo, Pagellus erythrinus and one species of Bothidae, Paralichthys olivaceus. Of the 68 loci screened, sixteen were found to be polymorphic when tested in 20 individual black sea bream, Acanthopagrus schlegeli. The number of alleles per locus ranged from 2 to 9, and the observed and expected heterozygosity ranged from 0.55 to 0.95, and from 0.58 to 0.87, respectively. Our results show that cross-species amplification of known microsatellite loci in closely related species is a highly promising source of microsatellite markers for A. schlegeli.