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Identification of the human beta-actin enhancer and its binding factor.

1988; Taylor & Francis; Volume: 8; Issue: 1 Linguagem: Inglês

10.1128/mcb.8.1.267

1098-5549

Takeshi Kawamoto, K Makino, Hitoshi Niwa, Haruo Sugiyama, S Kimura, M Amemura, Akio Nakata, Takeo Kakunaga,

Virus-based gene therapy research

An enhancer of the human beta-actin gene and a factor that specifically interacts with it were detected. A mobility shift assay showed that the factor bound to the 25-base-pair sequence (between +759 and +783 downstream from the cap site) with high specificity. This finding correlated with those of DNase I protection and exonuclease III digestion assays. This binding region of the beta-actin enhancer contained a hyphenated dyad symmetry and an enhancer core-like sequence. In vitro competition experiments indicated that the factor did not bind to the simian virus 40 enhancer core region.