Artigo Revisado por pares

Covalent Immobilization of Single Proteins on Mica for Molecular Recognition Force Microscopy

2003; Wiley; Volume: 4; Issue: 12 Linguagem: Inglês

10.1002/cphc.200300844

ISSN

1439-7641

Autores

Dionne C.G. Klein, Cordula M. Stroh, Henriette Jensenius, Maarten van Es, A. S. M. Kamruzzahan, Amalia Stamouli, Hermann J. Gruber, Tjerk H. Oosterkamp, Peter Hinterdorfer,

Tópico(s)

Polymer Surface Interaction Studies

Resumo

ChemPhysChemVolume 4, Issue 12 p. 1367-1371 Communication Covalent Immobilization of Single Proteins on Mica for Molecular Recognition Force Microscopy Dionne C. G. Klein, Dionne C. G. Klein Leiden Institute of Physics, Leiden University, Niels Bohrweg 2, 2333 CA Leiden, The NetherlandsSearch for more papers by this authorCordula M. Stroh Dr., Cordula M. Stroh Dr. Institute of Biophysics, University of Linz, Altenbergerstraße 69, University of Linz, 4040 Linz, Austria, Fax: (+43) 732-2468-822Search for more papers by this authorHenriette Jensenius Dr., Henriette Jensenius Dr. Leiden Institute of Physics, Leiden University, Niels Bohrweg 2, 2333 CA Leiden, The NetherlandsSearch for more papers by this authorMaarten van Es, Maarten van Es Leiden Institute of Physics, Leiden University, Niels Bohrweg 2, 2333 CA Leiden, The Netherlands Institute of Biophysics, University of Linz, Altenbergerstraße 69, University of Linz, 4040 Linz, Austria, Fax: (+43) 732-2468-822Search for more papers by this authorA. S. M. Kamruzzahan, A. S. M. Kamruzzahan Institute of Biophysics, University of Linz, Altenbergerstraße 69, University of Linz, 4040 Linz, Austria, Fax: (+43) 732-2468-822Search for more papers by this authorAmalia Stamouli Dr., Amalia Stamouli Dr. Leiden Institute of Physics, Leiden University, Niels Bohrweg 2, 2333 CA Leiden, The NetherlandsSearch for more papers by this authorHermann J. Gruber Prof., Hermann J. Gruber Prof. Institute of Biophysics, University of Linz, Altenbergerstraße 69, University of Linz, 4040 Linz, Austria, Fax: (+43) 732-2468-822Search for more papers by this authorTjerk H. Oosterkamp Prof., Tjerk H. Oosterkamp Prof. Leiden Institute of Physics, Leiden University, Niels Bohrweg 2, 2333 CA Leiden, The NetherlandsSearch for more papers by this authorPeter Hinterdorfer Prof., Peter Hinterdorfer Prof. [email protected] Institute of Biophysics, University of Linz, Altenbergerstraße 69, University of Linz, 4040 Linz, Austria, Fax: (+43) 732-2468-822Search for more papers by this author Dionne C. G. Klein, Dionne C. G. Klein Leiden Institute of Physics, Leiden University, Niels Bohrweg 2, 2333 CA Leiden, The NetherlandsSearch for more papers by this authorCordula M. Stroh Dr., Cordula M. Stroh Dr. Institute of Biophysics, University of Linz, Altenbergerstraße 69, University of Linz, 4040 Linz, Austria, Fax: (+43) 732-2468-822Search for more papers by this authorHenriette Jensenius Dr., Henriette Jensenius Dr. Leiden Institute of Physics, Leiden University, Niels Bohrweg 2, 2333 CA Leiden, The NetherlandsSearch for more papers by this authorMaarten van Es, Maarten van Es Leiden Institute of Physics, Leiden University, Niels Bohrweg 2, 2333 CA Leiden, The Netherlands Institute of Biophysics, University of Linz, Altenbergerstraße 69, University of Linz, 4040 Linz, Austria, Fax: (+43) 732-2468-822Search for more papers by this authorA. S. M. Kamruzzahan, A. S. M. Kamruzzahan Institute of Biophysics, University of Linz, Altenbergerstraße 69, University of Linz, 4040 Linz, Austria, Fax: (+43) 732-2468-822Search for more papers by this authorAmalia Stamouli Dr., Amalia Stamouli Dr. Leiden Institute of Physics, Leiden University, Niels Bohrweg 2, 2333 CA Leiden, The NetherlandsSearch for more papers by this authorHermann J. Gruber Prof., Hermann J. Gruber Prof. Institute of Biophysics, University of Linz, Altenbergerstraße 69, University of Linz, 4040 Linz, Austria, Fax: (+43) 732-2468-822Search for more papers by this authorTjerk H. Oosterkamp Prof., Tjerk H. Oosterkamp Prof. Leiden Institute of Physics, Leiden University, Niels Bohrweg 2, 2333 CA Leiden, The NetherlandsSearch for more papers by this authorPeter Hinterdorfer Prof., Peter Hinterdorfer Prof. [email protected] Institute of Biophysics, University of Linz, Altenbergerstraße 69, University of Linz, 4040 Linz, Austria, Fax: (+43) 732-2468-822Search for more papers by this author First published: 08 December 2003 https://doi.org/10.1002/cphc.200300844Citations: 30Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Graphical Abstract Tether them! A matrix of proteins that are covalently bound to mica with only one short tether per protein was created in order to fulfil all requirements for molecular recognition force microscopy of single proteins (see picture). This immobilization technique produces a high density of individual proteins on a flat surface in liquid with extremely low surface roughness. References 1 G. U. Lee, D. A. Kidwell, R. J. Colton, Langmuir 1994, 10, 354– 357. 2 E. L. Florin, V. T. Moy, H. E. Gaub, Science 1994, 264, 415– 417. 3 P. Hinterdorfer, W. Baumgartner, H. J. Gruber, K. Schilcher, H. Schindler, Proc. Natl. Acad. Sci. USA 1996, 93, 3477– 3481. 4 M. Grandbois, W. Dettmann, M. Benoit, H. E. Gaub, J. Histochem. Cytochem. 2000, 48, 719– 724. 5 O. H. Willemsen, M. M. E. Snel, K. O. van der Werf, B. G. de Grooth, J. Greve, P. Hinterdorfer, H. J. Gruber, H. Schindler, Y. van Kooyk, C. G. Figdor, Biophys. J. 1998, 75, 2220– 2228. 6 A. Raab, W. Han, D. Badt, S. J. Smith-Gill, S. M. Lindsay, H. Schindler, P. 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