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ABNORMALITIES OF SARCOPLASMIC RETICULUM Ca 2+ MOBILIZATION IN AORTIC SMOOTH MUSCLE CELLS FROM STREPTOZOTOCIN‐INDUCED DIABETIC RATS

2007; Wiley; Volume: 35; Issue: 5-6 Linguagem: Inglês

10.1111/j.1440-1681.2007.04832.x

1440-1681

Li Ma, Banghao Zhu, Xiangping Chen, Jie Liu, Yong‐Yuan Guan, Jun Ren,

Nitric Oxide and Endothelin Effects

Clinical and Experimental Pharmacology and PhysiologyVolume 35, Issue 5-6 p. 568-573 ABNORMALITIES OF SARCOPLASMIC RETICULUM Ca2+ MOBILIZATION IN AORTIC SMOOTH MUSCLE CELLS FROM STREPTOZOTOCIN-INDUCED DIABETIC RATS Li Ma, Li Ma Department of Pharmacology, Cardiac and Cerebral Vascular Research Center, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou, China andSearch for more papers by this authorBanghao Zhu, Banghao Zhu Department of Pharmacology, Cardiac and Cerebral Vascular Research Center, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou, China andSearch for more papers by this authorXiangping Chen, Xiangping Chen Department of Pharmacology, Cardiac and Cerebral Vascular Research Center, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou, China andSearch for more papers by this authorJie Liu, Jie Liu Department of Pharmacology, Cardiac and Cerebral Vascular Research Center, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou, China andSearch for more papers by this authorYongyuan Guan, Yongyuan Guan Department of Pharmacology, Cardiac and Cerebral Vascular Research Center, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou, China andSearch for more papers by this authorJun Ren, Jun Ren Center for Cardiovascular Research and Alternative Medicine, Division of Pharmaceutical Sciences, University of Wyoming, Laramie, Wyoming, USASearch for more papers by this author Li Ma, Li Ma Department of Pharmacology, Cardiac and Cerebral Vascular Research Center, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou, China andSearch for more papers by this authorBanghao Zhu, Banghao Zhu Department of Pharmacology, Cardiac and Cerebral Vascular Research Center, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou, China andSearch for more papers by this authorXiangping Chen, Xiangping Chen Department of Pharmacology, Cardiac and Cerebral Vascular Research Center, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou, China andSearch for more papers by this authorJie Liu, Jie Liu Department of Pharmacology, Cardiac and Cerebral Vascular Research Center, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou, China andSearch for more papers by this authorYongyuan Guan, Yongyuan Guan Department of Pharmacology, Cardiac and Cerebral Vascular Research Center, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou, China andSearch for more papers by this authorJun Ren, Jun Ren Center for Cardiovascular Research and Alternative Medicine, Division of Pharmaceutical Sciences, University of Wyoming, Laramie, Wyoming, USASearch for more papers by this author First published: 07 December 2007 https://doi.org/10.1111/j.1440-1681.2007.04832.xCitations: 17 Dr Bang-Hao Zhu, Department of Pharmacology, Cardiac and Cerebral Vascular Research Center, Zhongshan Medical College, Sun Yat-Sen University, No. 74 Zhongshan Road 2, Guangzhou, Guangdong 510080, China. Email: zhubh@mail.sysu.edu.cn Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat SUMMARY 1 Previously, we found that contractions in response to receptor-dependent (i.e. a1-adrenoceptor agonist phenylephrine) and -independent (i.e. cyclopiazonic acid) stimuli are decreased in rat aorta during late diabetes. The aim of the present study was to further investigate the changes of intracellular Ca2+ homeostasis in diabetic aortic smooth muscle cells. Functional changes of inositol 1,4,5-trisphosphate (IP3)- and ryanodine-sensitive Ca2+ stores of the sarcoplasmic reticulum (SR) were evaluated using Fluo-3 acetoxymethyl ester fluorescence, western blot and organ bath techniques. 2 In aortic smooth muscle cells from diabetic rats, the Ca2+ release and Ca2+ influx caused by both 10 mmol/L phenylephrine (depletion of IP3-sensitive Ca2+ stores) and 1 mmol/L ryanodine (depletion of ryanodine-sensitive Ca2+ stores) were both significantly decreased compared with control. Moreover, protein expression levels of IP3 (260 kDa) and ryanodine receptors (500 kDa) were reduced by 31.8 ± 7.7 and 69.2 ± 8.4%, respectively, in aortas from diabetic rats compared with those from control rats. 3 In diabetic rat aorta, phenylephrine-induced contractility was decreased to approximately two-thirds of that in controls, whereas ryanodine alone did not cause obvious contraction in aortas from either control or diabetic rats. 4 The present results suggest that the hyporeactivity of aortic smooth muscle to vasoconstrictors in diabetes results mainly from changes to the IP3-sensitive Ca2+ release pathway. The SR Ca2+ signalling pathway plays a crucial role in the development of diabetic vascular complications. Citing Literature Volume35, Issue5-6May/June 2008Pages 568-573 RelatedInformation