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Establishment, characterization, and viral susceptibility of two cell lines derived from goldfish Carassius auratus muscle and swim bladder

2007; Inter-Research; Volume: 77; Linguagem: Inglês

10.3354/dao01802

1616-1580

L. Rougée, GK Ostrander, RH Richmond, Yuanan Lu,

CRISPR and Genetic Engineering

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 77:127-135 (2007) - DOI: https://doi.org/10.3354/dao01802 Establishment, characterization, and viral susceptibility of two cell lines derived from goldfish Carassius auratus muscle and swim bladder Luc Rougée1, Gary K. Ostrander2, Robert H. Richmond1, Yuanan Lu3,* 1Kewalo Marine Laboratory, Pacific Biosciences Research Center, University of Hawaii at Manoa, 41 Ahui Street, Honolulu, Hawaii 96813, USA 2Pacific Biosciences Research Center, University of Hawaii at Manoa, 2500 Campus Road, Hawaii Hall 211, Honolulu, Hawaii 96822, USA 3Department of Public Health Sciences, University of Hawaii at Manoa, 1960 East West Road, Biomed D104, Honolulu, Hawaii 96822, USA *Corresponding author. Email: ylu@pbrc.hawaii.edu ABSTRACT: Goldfish Carassius auratus are common aquarium fish and have a significant economic and research value, having considerable worth to fisheries as a baitfish and the ability to adapt to a range of habitats. Two cell lines were established from goldfish muscle and swim bladder tissue, in order to create a biological monitoring tool for viral diseases. Cell lines were optimally maintained at 30°C in Leibovitz-15 medium supplemented with 20% fetal bovine serum. Propagation of goldfish cells was serum dependent, with a low plating efficiency (>16%). Karyotyping analysis indicated that both cell lines remained diploid, with a mean chromosomal count of 104. Results of viral challenge assays revealed that both cell lines shared similar patterns of viral susceptibility and production to infectious hematopoietic necrosis virus, infectious pancreatic necrosis virus, snakehead rhabdovirus, and spring viremia carp virus. Both cell lines demonstrated a higher sensitivity and significantly larger viral production than control brown bullhead cells for channel catfish virus. These newly established cell lines will be used as a diagnostic tool for viral diseases in this fish species and also for the isolation and study of goldfish viruses in the future. KEY WORDS: Cell lines · Carassius auratus · Goldfish · Viral susceptibility · Fish Full text in pdf format PreviousNextCite this article as: Rougée L, Ostrander GK, Richmond RH, Lu Y (2007) Establishment, characterization, and viral susceptibility of two cell lines derived from goldfish Carassius auratus muscle and swim bladder. Dis Aquat Org 77:127-135. https://doi.org/10.3354/dao01802 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 77, No. 2. Online publication date: September 14, 2007 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2007 Inter-Research.