Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle
2002; Elsevier BV; Volume: 84; Issue: 9 Linguagem: Inglês
10.1016/s0300-9084(02)01440-2
ISSN1638-6183
AutoresMarianna Teplova, Christopher J. Wilds, Z. Wawrzak, Valentina Tereshko, Quan Du, Nicolas Carrasco, Zhen Huang, Martin Egli,
Tópico(s)Enzyme Structure and Function
ResumoSelenium was incorporated into an oligodeoxynucleotide in the form of 2′-methylseleno-uridine (USe). The X-ray crystal structure of the duplex 〚d(GCGTA)USed(ACGC)〛2 was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 Å, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2′-α-position with selenium does not affect the preference of the sugar for the C3′-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.
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