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T cell lysis of murine renal cancer: multiple signaling pathways for cell death via Fas

2000; Oxford University Press; Volume: 68; Issue: 1 Linguagem: Inglês

10.1189/jlb.68.1.81

1938-3673

Thomas J. Sayers, Alan D. Brooks, Naoko Seki, Mark J. Smyth, Hideo Yagita∥, Bruce R. Blazar, Anatoli Malyguine,

Immunotherapy and Immune Responses

Abstract Activated T cells lyse the murine renal cancer Renca. We have examined the mechanism of tumor cell lysis with the use of T cells derived from C57BL/6, BALB/c, B6.gld, and B6.Pfp-/- mice. C57BL/6 and BALB/c T cells can lyse Renca cells through the use of both granule- and Fas ligand (FasL)-mediated pathways. However, B6.gld T cells predominantly use granule-mediated killing, whereas B6.Pfp-/- T cells use FasL. The lysis of Renca by Pfp-/- T cells is only partially inhibited by the caspase inhibitor ZVAD-FMK, suggesting that caspase-independent signaling is also important for Renca cell lysis. When the reactive oxygen scavenger butylated hydroxyanisole was used alone or in combination with ZVAD-FMK a substantial reduction of Renca lysis was observed. Therefore, the caspase-independent generation of reactive oxygen intermediates in Renca after Fas triggering contributes to the lysis of these cells.