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Hepatitis C virus infection protein network

2008; Springer Nature; Volume: 4; Issue: 1 Linguagem: Inglês

10.1038/msb.2008.66

1744-4292

Benoı̂t de Chassey, Vincent Navratil, Lionel Tafforeau, Marie–Sophie Hiet, Anne Aublin‐Gex, Sophie Agaugué, Grégory Meiffren, Fabrine Pradezynski, B F Faria, T Chantier, Marc Breton, Johann Pellet, Nathalie Davoust, Philippe Mangeot, Annie Chaboud, François Pénin, Yves Jacob, Pierre‐Olivier Vidalain, Marc Vidal, Patrice André, Chantal Rabourdin‐Combe, Vincent Lotteau,

Biochemical and Molecular Research

A proteome-wide mapping of interactions between hepatitis C virus (HCV) and human proteins was performed to provide a comprehensive view of the cellular infection. A total of 314 protein-protein interactions between HCV and human proteins was identified by yeast two-hybrid and 170 by literature mining. Integration of this data set into a reconstructed human interactome showed that cellular proteins interacting with HCV are enriched in highly central and interconnected proteins. A global analysis on the basis of functional annotation highlighted the enrichment of cellular pathways targeted by HCV. A network of proteins associated with frequent clinical disorders of chronically infected patients was constructed by connecting the insulin, Jak/STAT and TGFbeta pathways with cellular proteins targeted by HCV. CORE protein appeared as a major perturbator of this network. Focal adhesion was identified as a new function affected by HCV, mainly by NS3 and NS5A proteins.