Sodium dependent acetate formation from CO2 in Peptostreptococcus productus (strain Marburg)
1989; Oxford University Press; Volume: 57; Issue: 3 Linguagem: Inglês
10.1111/j.1574-6968.1989.tb03363.x
ISSN1574-6968
AutoresGabriele Geerligs, Peter Schönheit, Gabriele Diekert,
Tópico(s)Microbial Natural Products and Biosynthesis
ResumoWashed cells of Peptostreptococcus productus (strain Marburg), which were incubated in the presence of CO/CO2/N2 (50%/ 17%/ 33%; 200 kPa) catalyzed the synthesis of acetate from carbon monoxide. The rate of acetate formation from CO was stimulated more than threefold by the addition of sodium (10 mM); potassium did not effect acetate synthesis. The degree of stimulation was dependent on the sodium concentration; the dependence followed simple Michaelis-Menten kinetics. The apparent Km for sodium was determined to be about 2 mmol/1. Sodium also stimulated acetate synthesis from H2 plus CO2. In the absence of added sodium the formation of formate as an intermediate in methyl group synthesis was stimulated. It is suggested that the sodium dependent reaction(s) is one (or more) of the reactions involved in methyl group synthesis from CO2.
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