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Nitrogen-fixing and cellulose-producing Gluconacetobacter kombuchae sp. nov., isolated from Kombucha tea

2007; Microbiology Society; Volume: 57; Issue: 2 Linguagem: Inglês

10.1099/ijs.0.64638-0

1466-5034

Debasree Dutta, Ratan Gachhui,

Microbial Natural Products and Biosynthesis

A few members of the family Acetobacteraceae are cellulose-producers, while only six members fix nitrogen. Bacterial strain RG3 T , isolated from Kombucha tea, displays both of these characteristics. A high bootstrap value in the 16S rRNA gene sequence-based phylogenetic analysis supported the position of this strain within the genus Gluconacetobacter , with Gluconacetobacter hansenii LMG 1527 T as its nearest neighbour (99.1 % sequence similarity). It could utilize ethanol, fructose, arabinose, glycerol, sorbitol and mannitol, but not galactose or xylose, as sole sources of carbon. Single amino acids such as l -alanine, l -cysteine and l -threonine served as carbon and nitrogen sources for growth of strain RG3 T . Strain RG3 T produced cellulose in both nitrogen-free broth and enriched medium. The ubiquinone present was Q-10 and the DNA base composition was 55.8 mol% G+C. It exhibited low values of 5.2–27.77 % DNA–DNA relatedness to the type strains of related gluconacetobacters, which placed it within a separate taxon, for which the name Gluconacetobacter kombuchae sp. nov. is proposed, with the type strain RG3 T (=LMG 23726 T =MTCC 6913 T ).