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BIBTEX RIS

Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale

2005; Instituto Oswaldo Cruz, Ministério da Saúde; Volume: 100; Issue: 7 Linguagem: Inglês

10.1590/s0074-02762005000700016

ISSN

1678-8060

Autores

Flábio R. Araújo, Valeska SP Melo, Carlos AN Ramos, C. R. Madruga, Cleber O. Soares, Raul H. Kessler, Nalvo F. Almeida, Graziela S Araújo, Lêucio Câmara Alves, Roberto AA Torres Júnior, Stênio Perdigão Fragoso, Paulo R.C. Arauco, Gisele Bacanelli, Maristeli B Oliveira, Lenita Ramires dos Santos,

Tópico(s)

Trypanosoma species research and implications

Resumo

Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant MSP1a and MSP2 from a Brazilian isolate of Anaplasma marginale were developed to detect antibodies against this rickettsia in cattle. The high sensitivities (99% for both tests) and specificities (100% for both tests) were confirmed with sera from cattle positive or negative for A. marginale antibodies, respectively, by immunofluorescent antibody test. By the analysis of 583 sera from cattle of three regions of the state of Pernambuco, Brazil, the agreement between both tests was high, with a kappa index of 0.89. The similar performances of the ELISAs suggest that both tests can be used in epidemiological surveys for detection of antibodies to A. marginale in cattle.

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