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In vivo and in vitro differentiation of myocytes from human bone marrow–derived multipotent progenitor cells

2003; Elsevier BV; Volume: 31; Issue: 12 Linguagem: Inglês

10.1016/j.exphem.2003.09.003

1873-2399

Yukari Muguruma, Morayma Reyes, Yoshihiko Nakamura, Tadayuki Sato, Hideyuki Matsuzawa, Hiroko Miyatake, Akira Akatsuka, Johbu Itoh, Takashi Yahata, Kiyoshi Ando, Shunichi Kato, Tomomitsu Hotta,

Muscle Physiology and Disorders

ObjectiveRecent studies have shown that bone marrow (BM) contains cells capable of differentiating into myocytes in vivo. However, addition of demethylation drugs has been necessary to induce myocyte differentiation from BM cells in vitro, and precise mechanisms of BM cells' conversion to myocytes and the origin of those cells have not been established. We investigated the expression of myogenic markers during differentiation and maturation of myocytes from BM-derived multipotent adult progenitor cells (MAPC) under physiological culture condition.Materials and methodsFrozen BM samples from 21 healthy donors were used as a source of MAPC. To induce myocyte differentiation MAPC was cultured in the presence of 5% FCS, VEGF, bFGF, and IGF-1, and the expressions of myocyte markers were examined at various time points. We also investigated engraftment and differentiation of MAPC-derived myocytes in vivo.ResultsFrozen BM-derived MAPC, cultured under the physiological myogenic condition, demonstrated spatial expression patterns of several myocyte markers similar to that of authentic myocyte differentiation. When injected into murine muscles, MAPC treated with the myogenic condition engrafted and differentiated into myocyte marker–positive cells and myotubes in vivo.ConclusionFor the first time, we were able to induce myocyte formation from BM cells under the physiological condition in vitro and demonstrated that treating cells with this condition prior to intramuscular injection increased efficiency of engraftment and differentiation in vivo.