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Development of a Rapid Immunochromatographic Test for Simultaneous Serodiagnosis of Bovine Babesioses Caused by Babesia bovis and Babesia bigemina

2008; American Society of Tropical Medicine and Hygiene; Volume: 78; Issue: 1 Linguagem: Inglês

10.4269/ajtmh.2008.78.117

1476-1645

Chul-Min Kim, Lidia Beatriz Conza Blanco, Andy Alhassan, Hiroshi Iseki, Naoaki Yokoyama, Xuenan Xuan, Ikuo Igarashi,

Viral Infections and Vectors

With the objective of developing a simpler diagnostic alternative, a rapid immunochromatographic test (BoiICT) was constructed for the simultaneous detection of Babesia bovis - and Babesia bigemina -specific antibodies using B. bovis recombinant merozoite surface antigen-2c and B. bigemina recombinant rhoptry-associated protein-1. The BoiICT selectively detected specific antibodies to B. bovis and B. bigemina . All sera from cattle infected with other protozoan parasites (i.e., Cryptosporidium parvum, Neospora caninum, and Theileria orientalis ) showed negative results in the BoiICT. The relative sensitivity and specificity for detecting antibody to B. bovis were 96.7% (29 of 30) and 91.3% (73 of 80), respectively. The relative sensitivity and specificity for detecting antibody to B. bigemina were 96.7% (29 of 30) and 92.5% (74 of 80), respectively. These findings indicate that the BoiICT is useful for fast field diagnostic assessment of bovine babesioses without any laboratory equipment.