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HLA‐DC antigens can serve as recognition elements for human cytotoxic T lymphocytes

1984; Wiley; Volume: 14; Issue: 4 Linguagem: Inglês

10.1002/eji.1830140404

1521-4141

Hergen Spits, Jannie Borst, Marius J. Giphart, John E. Coligan, Cox Terhorst, J E de Vries,

Monoclonal and Polyclonal Antibodies Research

Abstract The specificity of four cytotoxic T lymphocyte (CTL) clones which recognize class II major histocompatibility complex (MHC) antigens was analyzed. All clones recognized antigens associated with the serologically defined HLA‐DRw6 specificity. The activity of two of these clones, JR‐2‐2 and JR‐2‐10, could be inhibited by a monoclonal antibody Q 5/13 specific for a monomorphic determinant present on HLA‐DR. In contrast, the activity of the two other CTL clones, JR‐2‐19 and JR‐2‐26, was not blocked by Q 5/13, but by a new monoclonal reagent, SPV‐L3. This latter monoclonal antibody precipitated a two‐chain structure of 28 kDa and 33 kDa and reacts with a monomorphic determinant. The molecular weight of the polypeptides precipitated with SPV‐L3 was slightly less than those precipitated with a HLA‐DR‐specific monoclonal reagent. In addition two‐dimensional gel electrophoresis showed that the antigen precipitated by SPV‐L3 differed in charge from those precipitated with the anti‐HLA‐DR antibody. These results indicate that SPV‐L3 recognizes a class II MHC product different from HLA‐DR. This observation was confirmed by partial amino acid sequence analysis of the two chains which revealed that the molecule precipitated by SPV‐L3 is homologous to HLA‐DC/DS molecules. Therefore this report provides the first evidence that human cytotoxic T cells can recognize HLA‐DC/DS antigens.