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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences

2002; National Academy of Sciences; Volume: 99; Issue: 26 Linguagem: Inglês

10.1073/pnas.242603899

1091-6490

Robert L. Strausberg, Elise A. Feingold, Lynette Grouse, Jeffery G. Derge, Richard D. Klausner, Francis S. Collins, Lukas Wagner, Carolyn M. Shenmen, Gregory D. Schuler, Stephen F. Altschul, Barry R. Zeebèrg, Kenneth H. Buetow, Carl F. Schaefer, Narayan Bhat, Ralph F. Hopkins, Heather Jordan, Troy Moore, Steve I Max, Jun Wang, Florence Y. Hsieh, Luda Diatchenko, Kate Marusina, Andrew Farmer, Gerald M. Rubin, Ling Hong, Mark Stapleton, Marcelo B. Soares, Maria F. Bonaldo, T.L. Casavant, Todd E. Scheetz, Michael Brownstein, Ted B. Usdin, Toshiyuki Shiraki, Piero Carninci, Christa Prange, Sam S Raha, Naomi A Loquellano, Garrick Peters, Rick D Abramson, Sara J Mullahy, Stephanie Bosak, Paul McEwan, Kevin McKernan, Joel A. Malek, Preethi H. Gunaratne, Stephen Richards, Kim C. Worley, Sarah Hale, Angela Garcia, Stephen W. Hulyk, Debbie K Villalon, Donna M. Muzny, Erica Sodergren, Xiuhua Lu, Richard A. Gibbs, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha Madan, Stephanie Rodrigues, Amy Sanchez, Michelle Whiting, Anup Madan, Alice Young, Yuriy Shevchenko, Gerard G. Bouffard, Robert W. Blakesley, Jeffrey W. Touchman, Eric D. Green, Mark Dickson, Álex Rodríguez, Jonathan Wood, Jeremy Schmutz, R Myers, Yaron S N Butterfield, Martin Krzywinski, Ursula Skalska, Duane E. Smailus, Angelique Schnerch, Jacqueline E. Schein, Steven J.M. Jones, Marco A Marra,

RNA and protein synthesis mechanisms

The National Institutes of Health Mammalian Gene Collection (MGC) Program is a multiinstitutional effort to identify and sequence a cDNA clone containing a complete ORF for each human and mouse gene. ESTs were generated from libraries enriched for full-length cDNAs and analyzed to identify candidate full-ORF clones, which then were sequenced to high accuracy. The MGC has currently sequenced and verified the full ORF for a nonredundant set of >9,000 human and >6,000 mouse genes. Candidate full-ORF clones for an additional 7,800 human and 3,500 mouse genes also have been identified. All MGC sequences and clones are available without restriction through public databases and clone distribution networks (see http://mgc.nci.nih.gov ).