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Inflammatory mediators expressed in human islets of Langerhans: implications for islet transplantation

2003; Elsevier BV; Volume: 308; Issue: 3 Linguagem: Inglês

10.1016/s0006-291x(03)01392-5

1090-2104

Ulrika Johansson, Annika Olsson, Susanne Gabrielsson, Bo Nilsson, Olle Korsgren,

Adenosine and Purinergic Signaling

Expression of immune modulating mediators in human Islets of Langerhans could have important implications for development of autoimmunity in type 1 diabetes and influence the outcome of clinical islet transplantation. Islets obtained from five donors were analyzed at various times after isolation using cDNA array technology. The Atlas Human Cytokine/Receptor and Hematology/Immunology nylon membranes representing 268 genes and 406, respectively, were used and the relative expression of each gene analyzed. Of the 51 gene products identified, high mRNA expression of MCP-1, MIF, VEGF, and thymosin β-10 was detected in all islet samples. IL-8, IL-1-β, IL-5R, and INF-γ antagonist were expressed in islets cultured for 2 days. IL-2R was expressed in islets cultured for more than 6 days. In conclusion, several inflammatory mediators were expressed in isolated islets, particularly at an early stage after isolation, indicating that a few days of culture could be beneficial for the outcome of islet transplantation.