Portal de Periódicos da CAPES

OSTEOPONTIN GENE EXPRESSION AND IMMUNOLOCALIZATION IN THE RABBIT URINARY TRACT

2002; Lippincott Williams & Wilkins; Volume: 167; Issue: 2 Part 1 Linguagem: Inglês

10.1016/s0022-5347(01)69138-9

1527-3792

Hwyda A. Arafat, Alan J. Wein, Samuel Chacko,

dental development and anomalies

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Feb 2002OSTEOPONTIN GENE EXPRESSION AND IMMUNOLOCALIZATION IN THE RABBIT URINARY TRACT H.A. ARAFAT, A.J. WEIN, and S. CHACKO H.A. ARAFATH.A. ARAFAT More articles by this author , A.J. WEINA.J. WEIN More articles by this author , and S. CHACKOS. CHACKO More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)69138-9AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Osteopontin is a highly phosphorylated, calcium binding sialoprotein characterized by a conserved arginine-glycine-aspartate sequence. Vitronectin receptor (αvβ3 integrin) and hyaluronan receptor (CD44) are documented as receptors for osteopontin and their expression has been established in the bladder. Based on that finding and the fact that osteopontin protein is present in urine we hypothesized that osteopontin is expressed in the lower urinary tract. Materials and Methods: Osteopontin messenger (m)RNA and protein were analyzed in 5 adult urinary tracts and 5 neonatal bladders of New Zealand White rabbits using reverse transcriptase-polymerase chain reaction and immunohistochemical testing. Analysis of mRNA expression and localization of osteopontin receptors, αvβ3 integrin and CD44 were also performed in adult bladders and primary cultures of detrusor myocytes. Results: Adult renal pelvis, ureter, bladder and urethra, and neonatal bladders contained significant levels of osteopontin mRNA. Immunohistochemical staining revealed osteopontin expression in all layers of the transitional epithelium of the bladder, co-localizing with αvβ3 integrin mainly in the superficial layers and with CD44 mainly in the basal layers. Osteopontin was detected within the cytoplasm of smooth muscle cells, while αvβ3 integrin was located closer to the plasmalemma. Furthermore, primary cultured detrusor myocytes expressed osteopontin mRNA in stable fashion for up to 4 passages. Treating bladder myocyte cultures with insulin-like growth factor-1 and 17β-estradiol resulted in up-regulation and down-regulation of osteopontin mRNA, respectively. Conclusions: Adult and neonatal rabbit detrusors are a prominent source of osteopontin in vivo and in vitro. Epithelial osteopontin may be a source of osteopontin in urine. The co-localization of osteopontin in the bladder epithelium with αvβ3 integrin and CD44 suggests a role in maintaining the integrity of the transitional epithelium by providing the sealing and adhesiveness needed for the impermeable state of the bladder. References 1 : The nature and significance of osteopontin. Connect Tissue Res1989; 23: 1231. Crossref, Google Scholar 2 : Isolation and characterization of two sialoproteins present only in bone calcified matrix. Biochem J1985; 232: 715. Crossref, Medline, Google Scholar 3 : Cloning and sequence analysis of rat bone sialoprotein (osteopontin) cDNA reveals an Arg-Gly-Asp cell-binding sequence. Proc Natl Acad Sci USA1986; 83: 8819. Crossref, Medline, Google Scholar 4 : Expression and distribution of osteopontin in human tissues: widespread association with luminal epithelial surfaces. Mol Biol Cell1992; 3: 1169. Crossref, Medline, Google Scholar 5 : Osteopontin expression and function: role in bone remodeling. J Cell Biochem1998; 30: 92. Crossref, Google Scholar 6 : Developmental expression of 2ar (osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridization. J Cell Biol1988; 106: 441. Crossref, Medline, Google Scholar 7 : Uropontin in urinary calcium stone formation. Miner Electrolyte Metab1994; 20: 385. Medline, Google Scholar 8 : Structure and expression of the mRNA encoding urinary stone protein (osteopontin). J Biol Chem1993; 268: 15180. Crossref, Medline, Google Scholar 9 : Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily. Proc Natl Acad Sci USA1992; 89: 426. Crossref, Medline, Google Scholar 10 : Macrophages express osteopontin during repair of myocardial necrosis. Am J Pathol1994; 145: 1450. Medline, Google Scholar 11 : Molecular cloning and characterization of 2B7, a rat mRNA which distinguishes smooth muscle cell phenotypes in vitro and is identical to osteopontin (secreted phosphoprotein I, 2aR). Biochem Biophys Res Comm1991; 177: 867. Crossref, Medline, Google Scholar 12 : Osteopontin expression in cardiovascular diseases. Ann NY Acad Sci1995; 760: 109. Crossref, Medline, Google Scholar 13 : Osteopontin promotes vascular cell adhesion and spreading and is chemotactic for smooth muscle cells in vitro. Circ Res1994; 74: 214. Crossref, Medline, Google Scholar 14 : Osteopontin-stimulated vascular smooth muscle cell migration is mediated by beta 3 integrin. Exp Cell Res1994; 214: 459. Crossref, Medline, Google Scholar 15 : Metanephric osteopontin regulates nephrogenesis in vitro. Am J Physiol1997; 272: F469. Medline, Google Scholar 16 : Receptor-ligand interaction between CD44 and osteopontin (Eta-1). Science1996; 271: 509. Crossref, Medline, Google Scholar 17 : Analysis of intracellular osteopontin as a marker of osteoblastic cell differentiation and mesenchymal cell migration. Eur J Oral Sci1998; 106: 401. Crossref, Medline, Google Scholar 18 : Intracellular osteopontin is an integral component of the CD44-ERM complex involved in cell migration. J Cell Physiol2000; 184: 118. Crossref, Medline, Google Scholar 19 : Extracellular matrix and integrin composition of the normal bladder wall. World J Urol1996; 14: S30. Crossref, Medline, Google Scholar 20 : The hyaluronic acid receptor is induced by stretch injury of rat bladder in vivo and influences smooth muscle cell contraction in vitro. J Urol1999; 162: 832. Link, Google Scholar 21 : Heterogeneity of bladder myocytes in vitro: modulation of myosin isoform expression. Tissue Cell2001; 33: 219. Crossref, Medline, Google Scholar 22 : Effect of estrogen on vascular smooth muscle cells is dependent upon cellular phenotype. Atherosclerosis1998; 140: 97. Crossref, Medline, Google Scholar 23 : Differentiated phenotype of smooth muscle cells depends on signaling pathways through insulin-like growth factors and phosphatidylinositol 3-kinase. J Biol Chem1998; 273: 28860. Crossref, Medline, Google Scholar 24 : Expression of wild-type and mutated rabbit osteopontin in Escherichia coli, and their effects on adhesion and migration of P388D1 cells. Biochem J1995; 307: 257. Crossref, Medline, Google Scholar 25 : Cloning of mouse integrin alphaV cDNA and role of the alphaV-related matrix receptors in metanephric development. J Cell Biol1996; 132: 1161. Crossref, Medline, Google Scholar 26 McHugh, K. P., Teitelbaum, S. L., Kitazawa, S. et al: Cloning and characterization of the murine beta3 integrin gene promoter: Identification of an interleukin-4 responsive element and regulation by Stat6. Gene Bank, Locus AF026509, 1998 Google Scholar 27 : CD44 is the principal cell surface receptor for hyaluronate. Cell1990; 61: 1303. Crossref, Medline, Google Scholar 28 : Expression of myosin isoforms in smooth muscle cells in the corpus cavernosum penis. Am J Physiol1998; 275: C976. Crossref, Medline, Google Scholar 29 : Osteopontin. Crit Rev Oral Biol Med2000; 11: 279. Crossref, Medline, Google Scholar 30 : Osteopontin expression in fetal and mature human kidney. J Am Soc Nephrol1999; 10: 444. Crossref, Medline, Google Scholar 31 : Osteopontin gene expression in the Holstein bull reproductive tract. J Androl2000; 21: 414. Medline, Google Scholar 32 : Osteopontin in urinary stone formation. Ann NY Acad Sci1995; 760: 257. Crossref, Medline, Google Scholar 33 : Renal cell osteopontin production is stimulated by calcium oxalate monohydrate crystals. Kidney Int1997; 51: 679. Crossref, Medline, Google Scholar 34 : Quantitative studies of human urinary excretion of uropontin. Kidney Int1998; 53: 189. Crossref, Medline, Google Scholar 35 : Adhesive properties of osteopontin: regulation by a naturally occurring thrombin-cleavage in close proximity to the GRGDS cell-binding domain. Mol Biol Cell1994; 5: 565. Crossref, Medline, Google Scholar 36 : Osteopontin N-terminal domain contains a cryptic adhesive sequence recognized by alpha9beta1integrin. J Biol Chem1996; 271: 28485. Crossref, Medline, Google Scholar 37 : Expression and in vitro regulation of integrins by normal human urothelial cells. Cell Adhes Comm1995; 3: 231. Crossref, Medline, Google Scholar 38 : The hyaluronate receptor is preferentially expressed on proliferating epithelial cells. J Cell Biol1989; 108: 1557. Crossref, Medline, Google Scholar 39 : CD44 cell adhesion molecules. Mol Pathol1999; 52: 189. Crossref, Medline, Google Scholar 40 : Integrins: cell adhesives and modulators of cell function. Histochem J1993; 25: 469. Crossref, Medline, Google Scholar 41 : Integrin signaling. Semin Cancer Biol1996; 7: 111. Crossref, Medline, Google Scholar 42 : Patterns of splice variant CD44 expression by normal human urothelium in situ and in vitro and by bladder-carcinoma cell lines. Int J Cancer1995; 62: 449. Crossref, Medline, Google Scholar 43 : Cellular adhesion molecules in urologic malignancies. Am J Clin Pathol1997; 107: 56. Crossref, Medline, Google Scholar 44 : Progressive loss of CD44 gene expression in invasive bladder cancer. Am J Pathol1996; 149: 873. Medline, Google Scholar 45 : Osteopontin overexpression is associated with arterial smooth muscle cell proliferation in vitro. Arterioscler Thromb1993; 13: 120. Crossref, Medline, Google Scholar 46 : Osteopontin is elevated during neointima formation in rat arteries and is a novel component of human atherosclerotic plaques. J Clin Invest1993; 92: 1686. Crossref, Medline, Google Scholar 47 : Osteopontin is produced by rat cardiac fibroblasts and mediates A(II)-induced DNA synthesis and collagen gel contraction. J Clin Invest1996; 98: 2218. Crossref, Medline, Google Scholar 48 : Myocardial osteopontin expression is associated with left ventricular hypertrophy. Circulation1997; 96: 3063. Crossref, Medline, Google Scholar 49 : Myocardial osteopontin expression coincides with the development of heart failure. Hypertension1999; 33: 663. Crossref, Medline, Google Scholar 51 : Direct interaction of filamin (ABP-280) with the beta 2-integrin subunit CD18. J Immunol1995; 154: 3461. Crossref, Medline, Google Scholar 52 : Role of osteopontin in cellular signaling and toxicant injury. Annu Rev Pharmacol Toxicol2001; 41: 723. Crossref, Medline, Google Scholar 53 : Signal transduction by G-proteins, rho-kinase and protein phosphatase to smooth muscle and non-muscle myosin II. J Physiol2000; 522: 177. Crossref, Medline, Google Scholar 54 : Molecular mechanisms of phenotypic plasticity in smooth muscle cells. J Appl Physiol2001; 90: 358. Crossref, Medline, Google Scholar 55 : Rho-Rho-kinase pathway in smooth muscle contraction and cytoskeletal reorganization of non-muscle cells. Trends Pharmacol Sci2001; 22: 32. Crossref, Medline, Google Scholar 56 : Role of intracellular calcium ([Ca2+]i) and tyrosine phosphorylation in adhesion of cultured vascular smooth muscle cells to fibrinogen. Cardiovasc Res1998; 39: 475. Crossref, Medline, Google Scholar 57 : Estrogen enhances differentiation of osteoblasts in mouse bone marrow culture. Bone1998; 22: 201. Crossref, Medline, Google Scholar 58 : Growth factors regulate expression of osteoblast-associated genes. J Periodontol1999; 70: 1345. Crossref, Medline, Google Scholar 59 : Targeted overexpression of IGF-I evokes distinct patterns of organ remodeling in smooth muscle cell tissue beds of transgenic mice. J Clin Invest1997; 100: 1425. Crossref, Medline, Google Scholar 60 : Blocking ligand occupancy of the alphaVbeta3 integrin inhibits insulin-like growth factor I signaling in vascular smooth muscle cells. Proc Natl Acad Sci USA1998; 95: 11217. Crossref, Medline, Google Scholar From the Division of Urology and Department of Pathobiology, University of Pennsylvania, Philadelphia, Pennsylvania© 2002 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited by Soriano A, Andy U, Hassani D, Whitmore K, Harvie H, Malykhina A and Arya L (2020) Relationship of Bladder Pain With Clinical and Urinary Markers of Neuroinflammation in Women With Urinary Urgency Without Urinary IncontinenceFemale Pelvic Medicine & Reconstructive Surgery, 10.1097/SPV.0000000000000951, VOL. 27, NO. 2, (e418-e422), Online publication date: 1-Feb-2021. Guelfi G, Stefanetti V, Zampini D, Oommen O, Brecchia G, Dall’Aglio C, Arcelli R, Cochetti G, Boni A and Mearini E (2017) Gold nanoparticles approach to detect chondroitin sulphate and hyaluronic acid urothelial coatingScientific Reports, 10.1038/s41598-017-09872-0, VOL. 7, NO. 1 de Almeida Prado P, Soares M, Lima F, Schor N and Teixeira V (2012) Amitriptyline aggravates the fibrosis process in a rat model of infravesical obstructionInternational Journal of Experimental Pathology, 10.1111/j.1365-2613.2012.00813.x, VOL. 93, NO. 3, (218-224), Online publication date: 1-Jun-2012. Ke H, Chang L, Yang S, Lin H, Li C, Wu D and Wu W (2011) Osteopontin overexpression predicts poor prognosis of upper urinary tract urothelial carcinomaUrologic Oncology: Seminars and Original Investigations, 10.1016/j.urolonc.2009.10.009, VOL. 29, NO. 6, (703-709), Online publication date: 1-Nov-2011. Aitken K and Bägli D (2009) The bladder extracellular matrix. Part I: architecture, development and diseaseNature Reviews Urology, 10.1038/nrurol.2009.201, VOL. 6, NO. 11, (596-611), Online publication date: 1-Nov-2009. Kunii Y, Niwa S, Hagiwara Y, Maeda M, Seitoh T and Suzuki T (2009) The immunohistochemical expression profile of osteopontin in normal human tissues using two site-specific antibodies reveals a wide distribution of positive cells and extensive expression in the central and peripheral nervous systemsMedical Molecular Morphology, 10.1007/s00795-009-0459-6, VOL. 42, NO. 3, (155-161), Online publication date: 1-Sep-2009. Arafat H, Katakam A, Chipitsyna G, Gong Q, Vancha A, Gabbeta J and Dafoe D (2007) Osteopontin Protects the Islets and β-Cells from Interleukin-1 β-Mediated Cytotoxicity through Negative Feedback Regulation of Nitric OxideEndocrinology, 10.1210/en.2006-0970, VOL. 148, NO. 2, (575-584), Online publication date: 1-Feb-2007. Taylor J, Zhu Q, Irwin B, Maghaydah Y, Tsimikas J, Pilbeam C, Leng L, Bucala R and Kuchel G (2006) Null mutation in macrophage migration inhibitory factor prevents muscle cell loss and fibrosis in partial bladder outlet obstructionAmerican Journal of Physiology-Renal Physiology, 10.1152/ajprenal.00144.2006, VOL. 291, NO. 6, (F1343-F1353), Online publication date: 1-Dec-2006. Hosoya A, Hoshi K, Sahara N, Ninomiya T, Akahane S, Kawamoto T and Ozawa H (2005) Effects of fixation and decalcification on the immunohistochemical localization of bone matrix proteins in fresh-frozen bone sectionsHistochemistry and Cell Biology, 10.1007/s00418-005-0791-4, VOL. 123, NO. 6, (639-646), Online publication date: 1-Jun-2005. Adwan H, Bäuerle T and Berger M (2003) Downregulation of osteopontin and bone sialoprotein II is related to reduced colony formation and metastasis formation of MDA-MB-231 human breast cancer cellsCancer Gene Therapy, 10.1038/sj.cgt.7700659, VOL. 11, NO. 2, (109-120), Online publication date: 1-Feb-2004. Volume 167Issue 2 Part 1February 2002Page: 746-752 Advertisement Copyright & Permissions© 2002 by American Urological Association, Inc.Keywordsrabbitsmuscle, smoothurinary tractbladdersialoglycoproteinsMetricsAuthor Information H.A. ARAFAT More articles by this author A.J. WEIN More articles by this author S. CHACKO More articles by this author Expand All Advertisement PDF downloadLoading ...