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Structure of the HIV-1 RNA packaging signal

2015; American Association for the Advancement of Science; Volume: 348; Issue: 6237 Linguagem: Inglês

10.1126/science.aaa9266

1095-9203

Sarah C. Keane, Xiao Heng, Kun Lu, Siarhei Kharytonchyk, Venkateswaran Ramakrishnan, Gregory Carter, Shawn Barton, Azra Hosic, Alyssa Florwick, Justin L C Santos, Nicholas C. Bolden, Sayo McCowin, David A. Case, Bruce A. Johnson, Marco Salemi, Alice Telesnitsky, Michael F. Summers,

Bacteriophages and microbial interactions

Structural signals that direct HIV packaging During the viral replication cycle of HIV, unspliced dimeric RNA genomes are efficiently packaged into new virions at the host cell membrane. Packaging is directed by a region at the start of the genome, the 5′ leader. The architecture of the 5′ leader remains controversial. Keane et al. developed nuclear magnetic resonance methods to determine the structure of a 155-nucleotide-long region of the 5′ leader that can direct viral packaging. The structure shows how the 5′ leader binds to the HIV protein that directs packaging, how unspliced dimeric genomes are selected for packaging, and how translation is suppressed when the genome dimerizes. Science , this issue p. 917