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Cell Cycle Regulators p105, p107, Rb2/p130, E2F4, p21CIP1/WAF1, Cyclin A in Predicting Cervical Intraepithelial Neoplasia, High-Risk Human Papillomavirus Infections and Their Outcome in Women Screened in Three New Independent States of the Former Soviet Union

2006; American Association for Cancer Research; Volume: 15; Issue: 7 Linguagem: Inglês

10.1158/1055-9965.epi-06-0086

1538-7755

Rosa Santopietro, Irena Shabalova, Nicolay Petrovichev, В. П. Козаченко, Tatjana Zakharova, Julia Pajanidi, Jurij Podistov, G. Yu. Chemeris, Larisa G. Sozaeva, Elena Lipova, Irena Tsidaeva, Olga Ivanchenko, Alla Pshepurko, Sergej Zakharenko, Raisa Nerovjna, Ludmila Kljukina, Oksana Erokhina, Marina Branovskaja, Maritta Ņikitina, Valerija Grunberga, Alexandr Grunberg, Anna Juschenko, Marcella Cintorino, Piero Tosi, Karí Syrjänen, Stina Syrjänen,

Molecular Biology Techniques and Applications

Abstract Background: The growth-controlling functions of the high-risk human papillomaviruses (HPV) depend on their ability to interact with several cellular proteins, including the key regulatory proteins of the cell cycle. We have examined the value of cell cycle regulatory proteins as predictors of the intermediate end point markers in cervical carcinogenesis: (a) grade of cervical intraepithelial neoplasia (CIN), (b) high-risk HPV type, (c) clearance/persistence of high-risk HPV, and (d) disease outcome in women participating in a multicenter follow-up study in three New Independent States countries. Methods: Totally, 232 biopsy samples tested high-risk HPV-positive and/or Papanicolaou smear–positive women were immunohistochemically stained for the following cell cycle markers: p105, p107, p130, E2F4, p21CIP1/WAF1/SDI1, cyclin A, and Ki-67. In addition, apoptotic index (AI) and mitotic index (MI) were determined in H&E-stained sections. Prospective follow-up data were available to disclose the clinical and virological outcome of the lesions. Results: The expression of Ki-67, p21CIP1/WAF1/SDI1, and cyclin A and AI and MI values were markedly increased in high-grade lesions, but only MI was an independent predictor of CIN3 in multivariate analysis. Cyclin A was the only independent predictor of high-risk HPV (odds ratio, 1.09; 95% confidence interval, 1.01-1.18; P = 0.021), exceeding the predictive power of CIN grade and high-grade squamous intraepithelial lesion Papanicolaou smears. None of these markers provided any useful predictive information as to the clinical and virological outcomes during the follow-up. Highly significant correlations (P = 0.0001) were found between AI and MI as well as between MI and cyclin A, Ki-67 and p21CIP1/WAF1/SDI1, Ki-67 and cyclin A, and p21CIP1/WAF1/SDI1 and cyclin A followed by that between p105 and cyclin A (P = 0.001) and p105 and p130 (P = 0.002). Conclusions: All tested factors related to cell cycle were increased, but only MI and cyclin A was an independent predictor of CIN3 and high-risk HPV carriage, respectively. (Cancer Epidemiol Biomarkers Prev 2006;15(7):1250–6)