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Detection of Human Cytomegalovirus DNA by Real-Time Quantitative PCR

2000; American Society for Microbiology; Volume: 38; Issue: 7 Linguagem: Inglês

10.1128/jcm.38.7.2734-2737.2000

1098-660X

Andreas Nitsche, Nina Steuer, Christian Schmidt, Olfert Landt, Heinz Ellerbrok, Georg Pauli, W. Siegert,

Parvovirus B19 Infection Studies

A real-time PCR assay was developed to quantify human cytomegalovirus (CMV) DNA. This assay was used to demonstrate a higher CMV DNA load in plasma of bone marrow transplant patients than in that of blood donors. The CMV load was higher in CMV antigen-positive patients than in antigen-negative patients.