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A Biological Porin Engineered into a Molecular, Nanofluidic Diode

2007; American Chemical Society; Volume: 7; Issue: 9 Linguagem: Inglês

10.1021/nl0716808

1530-6992

Henk Miedema, Maarten Vrouenraets, Jenny Wierenga, Wim Meijberg, George T. Robillard, Bob Eisenberg,

Membrane-based Ion Separation Techniques

We changed the nonrectifying biological porin OmpF into a nanofluidic diode. To that end, we engineered a pore that possesses two spatially separated selectivity filters of opposite charge where either cations or anions accumulate. The observed current inhibition under applied reverse bias voltage reflects, we believe, the creation of a zone depleted of charge carriers, in a sense very similar to what happens at the np junction of a semiconductor device.