Removing T-cell epitopes with computational protein design
2014; National Academy of Sciences; Volume: 111; Issue: 23 Linguagem: Inglês
10.1073/pnas.1321126111
ISSN1091-6490
AutoresChris King, Esteban N. Garza, Ronit Mazor, Jonathan L. Linehan, Ira Pastan, Marion Pepper, David Baker,
Tópico(s)Transgenic Plants and Applications
ResumoImmune responses can make protein therapeutics ineffective or even dangerous. We describe a general computational protein design method for reducing immunogenicity by eliminating known and predicted T-cell epitopes and maximizing the content of human peptide sequences without disrupting protein structure and function. We show that the method recapitulates previous experimental results on immunogenicity reduction, and we use it to disrupt T-cell epitopes in GFP and Pseudomonas exotoxin A without disrupting function.
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