Artigo Revisado por pares

The role of nucleophile-electrophile interactions in the unimolecular and bimolecular gas-phase ion chemistry of peptides and related systems

2000; Wiley; Volume: 35; Issue: 12 Linguagem: Inglês

10.1002/1096-9888(200012)35

ISSN

1096-9888

Autores

Richard A. J. O’Hair,

Tópico(s)

Advanced Proteomics Techniques and Applications

Resumo

This account describes the experimental tools (multi-stage mass spectrometric experiments, isotopic and structural labelling, kinetics and theoretical modelling) and physical organic concepts (influence of charge, the intermediacy of ion-molecule complexes, etc.) that can be used to unravel the mechanisms of gas-phase unimolecular and bimolecular ionic reactions of peptides. The role that nucleophile-electrophile interactions play in charge-directed reactions is highlighted for both unimolecular fragmentations (examples are illustrated for protonated sulfur-containing amino acids and peptides) and bimolecular ion-molecule reactions which cleave peptide bonds.

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