Portal de Periódicos da CAPES

Molecular Profiling Reveals Prognostically Significant Subtypes of Canine Lymphoma

2012; SAGE Publishing; Volume: 50; Issue: 4 Linguagem: Inglês

10.1177/0300985812465325

1544-2217

Aric M. Frantz, Aaron L. Sarver, Daisuke Ito, Tzu Phang, Anis Karimpour‐Fard, Milcah C. Scott, V. E. Valli, Kerstin Lindblad‐Toh, Kristine Burgess, Brian D. Husbands, Michael S. Henson, Antonella Borgatti, William C. Kisseberth, Lawrence Hunter, Matthew Breen, Timothy D. O’Brien, Jaime F. Modiano,

Virus-based gene therapy research

We performed genomewide gene expression analysis of 35 samples representing 6 common histologic subtypes of canine lymphoma and bioinformatics analyses to define their molecular characteristics. Three major groups were defined on the basis of gene expression profiles: (1) low-grade T-cell lymphoma, composed entirely by T-zone lymphoma; (2) high-grade T-cell lymphoma, consisting of lymphoblastic T-cell lymphoma and peripheral T-cell lymphoma not otherwise specified; and (3) B-cell lymphoma, consisting of marginal B-cell lymphoma, diffuse large B-cell lymphoma, and Burkitt lymphoma. Interspecies comparative analyses of gene expression profiles also showed that marginal B-cell lymphoma and diffuse large B-cell lymphoma in dogs and humans might represent a continuum of disease with similar drivers. The classification of these diverse tumors into 3 subgroups was prognostically significant, as the groups were directly correlated with event-free survival. Finally, we developed a benchtop diagnostic test based on expression of 4 genes that can robustly classify canine lymphomas into one of these 3 subgroups, enabling a direct clinical application for our results.