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BIBTEX RIS

Antibodies are not required to a protective immune response against dengue virus elicited in a mouse encephalitis model

2015; Elsevier BV; Volume: 487; Linguagem: Inglês

10.1016/j.virol.2015.10.006

1096-0341

Jaime Henrique Amorim, Rúbens Prince dos Santos Alves, Raíza Sales Pereira Bizerra, Sara Araújo Pereira, Lennon Ramos Pereira, Denicar Lina Nascimento Fabris, Robert Andreata‐Santos, Camila Malta Romano, Luís Carlos de Souza Ferreira,

Malaria Research and Control

Generating neutralizing antibodies have been considered a prerequisite to control dengue virus (DENV) infection. However, T lymphocytes have also been shown to be important in a protective immune state. In order to investigate the contribution of both humoral and cellular immune responses in DENV immunity, we used an experimental model in which a non-lethal DENV2 strain (ACS46) is used to intracranially prime Balb/C mice which develop protective immunity against a lethal DENV2 strain (JHA1). Primed mice generated envelope-specific antibodies and CD8+ T cell responses targeting mainly non-structural proteins. Immune sera from protected mice did not confer passive protection to naïve mice challenged with the JHA1 strain. In contrast, depletion of CD4+ and CD8+ T lymphocytes significantly reduced survival of ACS46-primed mice challenged with the JHA1 strain. Collectively, results presented in this study show that a cellular immune response targeting non-structural proteins are a promising way in vaccine development against dengue.