Comparison of Direct Rapid Tests for the Detection of Adenovirus Antigen in Routine Conjunctival Specimens
1989; Elsevier BV; Volume: 96; Issue: 7 Linguagem: Inglês
10.1016/s0161-6420(89)32793-x
ISSN1549-4713
AutoresRegis P. Kowalski, Y. J. Gordon,
Tópico(s)Parvovirus B19 Infection Studies
ResumoAbstract Two direct rapid tests to detect adenovirus antigen, enzyme immunoassay (EIA) and immunofiltration (IF) were compared with regard to sensitivity, specificity, ease of interpretation, and technical complexity against 75 adenovirus culture-positive and 35 adenovirus culture-negative conjunctival swab specimens. Enzyme immunoassay and IF were equally sensitive ( P = 0.5), with sensitivities of 80.6 and 79.0%, respectively, when swab specimens were collected from patients within 7 days of the onset of clinical symptoms of adenovirus infection. After 7 days of clinical onset, IF (46.2%) was more sensitive ( P < 0.01) than EIA (0.0%), and was able to detect residual antigen in three culture-negative specimens. Enzyme immunoassay and IF were equally specific (100%). Both tests were easy to interpret, but IF was more technically complex and is not yet commercially packaged. Enzyme immunoassay could benefit either an office practice or a central laboratory, whereas IF is better suited for the latter.
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