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Distribución de patrones PRA en aislamientos clínicos del complejo Mycobacterium avium procedentes de España y Suramérica.

2004; National Institute of Health; Volume: 24; Linguagem: Inglês

10.7705/biomedica.v24isupp1.1303

2590-7379

Martha Isabel Murcia, Sylvia Cardoso Leão, Viviana Ritacco, Elia Palenque, Rosângela Siqueira de Oliveira, Ana Reniero, M. Carmen Menéndez, María Alice da Silva Telles, David Jamil Hadad, Lucía Barrera, María Jesús García García,

Tuberculosis Research and Epidemiology

Mycobacterium avium complex (MAC) infections are the most frequent systemic infections associated with advanced AIDS. DNA probes for accurate identification of mycobacteria are available but are very expensive in many Latin American settings. Consequently, most Latin American diagnostic laboratories employ inaccurate and outdated tests for mycobacteria identification. Therefore, PCR restriction analysis (PRA) of the hsp65 gene was evaluated for the identification of 163 MAC human isolates originated from Spain and South America. The predominant PRA type in each country was: M. avium type I in Argentina (23/42, 55%) and Brazil (48/72, 67%), M. avium type II in Spain (18/26, 69%) and M. avium type III in Colombia (10/23, 43%). The Colombia frequency is noteworthy, since the PRA type III was quite infrequent in the other three countries. Furthermore, its presence has not been reported outside the Americas. The advantages and disadvantages of PRA in diagnostic mycobacteriology are discussed.