Genes misregulated in C. elegans deficient in Dicer, RDE-4, or RDE-1 are enriched for innate immunity genes
2007; Cold Spring Harbor Laboratory Press; Volume: 13; Issue: 7 Linguagem: Inglês
10.1261/rna.542107
ISSN1469-9001
AutoresNoah C. Welker, Jeffrey W. Habig, Brenda Bass,
Tópico(s)Genomics, phytochemicals, and oxidative stress
ResumoWe describe the first microarray analysis of a whole animal containing a mutation in the Dicer gene. We used adult Caenorhabditis elegans and, to distinguish among different roles of Dicer, we also performed microarray analyses of animals with mutations in rde-4 and rde-1, which are involved in silencing by siRNA, but not miRNA. Surprisingly, we find that the X chromosome is greatly enriched for genes regulated by Dicer. Comparison of all three microarray data sets indicates the majority of Dicer-regulated genes are not dependent on RDE-4 or RDE-1, including the X-linked genes. However, all three data sets are enriched in genes important for innate immunity and, specifically, show increased expression of innate immunity genes.
Referência(s)