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Amino Acid Sequences of the α and β Chains of Adult Hemoglobin of the European Hedgehog, Erinaceus europaeus1

1979; Oxford University Press; Volume: 85; Issue: 1 Linguagem: Inglês

10.1093/oxfordjournals.jbchem.a132319

1756-2651

Tetsuo Maita, Aiko Araya, Genji Matsuda, Morris Goodman,

Mass Spectrometry Techniques and Applications

Globin prepared from hemoglobin of the European hedgehog (Erinaceus europaeus) was separated into alpha and beta polypeptide chains by chromatography on a CM 52 column. The S-aminoethylated alpha and beta chains were each digested with trypsin and the resulting peptides were isolated. The sequences of all the tryptic peptides were established. The ordering of these peptides in the alpha and beta chains was deduced from their homology with the primary structures of alpha and beta chains of human adult hemoglobin. Comparing the primary structures of the alpha and beta chains of adult hemoglobin of the European hedgehog thus obtained with those of adult hemoglobin of the tupai (Tupaia glis), 35 amino acids substitutions in the alpha chains and 30 in the beta chains were recognized.