Label-Free DNA Biosensors Based on Functionalized Carbon Nanotube Field Effect Transistors
2009; American Chemical Society; Volume: 9; Issue: 2 Linguagem: Inglês
10.1021/nl8025604
ISSN1530-6992
AutoresMaria Teresa Martínez, Yu‐Chih Tseng, Nerea Ormategui, Iraida Loinaz, Ramón Eritja, Jeffrey Bokor,
Tópico(s)Advanced biosensing and bioanalysis techniques
ResumoA carbon nanotube transistor array was used to detect DNA hybridization. A new approach to ensure specific adsorption of DNA to the nanotubes was developed. The polymer poly (methylmethacrylate0.6-co-poly(ethyleneglycol)methacrylate0.15-co-N-succinimidyl methacrylate0.25) was synthesized and bonded noncovalently to the nanotube. Aminated single-strand DNA was then attached covalently to the polymer. After hybridization, statistically significant changes were observed in key transistor parameters. Hybridized DNA traps both electrons and holes, possibly caused by the charge-trapping nature of the base pairs.
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